Figure 1: Calibration curves of an ELISA that was either blocked only with BSA (left panel) or blocked with BSA and additionally stabilized with Liquid Plate Sealer® (right panel). Calibration curves at the starting day are identical. After 84 days at 37℃, no remaining binding activity of the capture antibodies is detectable in the unstabilized plate. In contrast, the stabilized plate does not show a significant decrease in sensitivity even after 84 days of incubation at 37℃
Figure 2: High-speed ELISA plate production with Liquid Plate Sealer® as blocking reagent and coating stabilizer in a single process step is suitable for sandwich ELISA and competitive tests. In serological assay formats however, it is sometimes better to use a more classical process as shown in the first row. In any case, washing after blocking can be commonly skipped when using Liquid Plate Sealer®.