丁香通

[40]Yan P S, Chen C M, Shi H, et al. Dissecting comples epigenetic alterations in breast cancer using CpG island microarrays [J]. Cancer Res, 2001, 61(23): 8375-8380.
[41]Huang T H, Perry M R, Laux D E. Methylation profiling of CpG islands in human breast cancer cells [J]. Hum Mol Genet, 1999, 8: 459-470.
[42]Gitan R S, Shi H, Chen C M, et al. Methylation-specific oligonucleotide microarray: a new potential for high-throughput methylation analysis [J]. Genome Res, 2002, 12: 158–164.
[43]Clement G., Benhattar J. A methylation sensitive dot blot assay (MS-DBA) for the quantitative analysis of DNA methylation in clinical samples [J]. Clin Pathol, 2005, Feb. 58(2): 155-8.
[44]Schouten J P, McElgunn C J, Waaijer R, et al. Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification [J]. Nucleic Acids Res, 2002, Jun 15, 30 (12): e57.
[45]Nygren A O, Ameziane N, Duarte H M, et al. Methylation-specific MLPA (MS-MLPA): simultaneous detection of CpG methylation and copy number changes of up to 40 sequences [J]. Nucleic Acids Res, 2005, Aug 16, 33 (14): e128.
[46]Costello J F, Fruhwald M C, Smiraglia D J, et al. Aberrant CpG-island methylation has non-random and tumour-type-specific patterns [J]. Nat Genet, 2000, Feb, 24(2): 132-8.
[47]Huang T H, Laux D E, Hamlin B C, et al. Identification of DNA methylation markers for human breast carcinomas using the methylation-sensitive restriction fingerprinting technique [J]. Cancer Res, 1997, Mar 15, 57(6): 1030-4.
[48]Frigola J, Ribas M, Risques R A, et al. Methylome profiling of cancer cells by amplification of inter-methylated sites (AIMS). Nucleic Acids Res, 2002, Apr 1. 30(7): e28.
[49]Hatada I, Hayashizaki Y, Hirotsune S, et al. A genomic scanning method for higher organisms using restriction sites as landmarks [J]. Proc Natl Acad Sci USA, 1991, 88: 9523-9527.
[50]Shiraishi M, Sekiguchi A, Oates A J, et al. Methyl-CpG binding domain column chromatography as a tool for the analysis of genomic DNA methylation. Ana Biochem, 2004, Jun 1, 329(1): 1-10.
[51]Meehan R R, Lewis J D, McKay S, et al. Identification of a mammalian protein that binds speciWcally to DNA containing methylated CpGs [J]. Cell, 1989, 58: 499-507.
[52]Bird A. DNA methylation patterns and epigenetic memory [J]. Genes Dev, 2002, 16: 6-21.
[53]Bird A P, Wolffe A P. Methylation-induced repression-belts, braces, and chromatin [J]. Cell, 1999, 99: 451-454.
[54]Lewis J D, Meehan R R, Henze W J, et al. Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA [J] Cell, 1992, 69: 905-914.
[55]Yegnasubramanian S, Lin X, Haffner M C, et al. Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative detection of DNA methylation [J]. Nucleic Acids Res, 2006, Feb 9, 34(3): e19.
[56]Hendrich B, Bird A. Identification and characterization of a family of mammalian methyl-CpG binding proteins [J]. Mol Cell Biol, 1998, 18: 6538–6547.
Review and estimation of methylation technology
GU Ting-Ting1.2, ZHANG Zhong-Ming1, ZHENG Peng-Sheng1.2.3
1. Department of Gynecology and Obstetrics, the First Hospital of Xi’an Jiaotong University Medical School, Shaanxi Xi’an 710061, China
2. Key Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University Medical School, Ministry of Education
3. Correspondence Author

Abstract: DNA methylation, a very important part in epigenetics, has been playing a pivotal role in retaining normal cell functions, gene imprinting, embryo development, and human tumorigenesis, and now is one of hot area in medical researches. With increasing progresses in the methylation studies, a series of detecting techniques has been developed to match various requirements of methylation studies. All of these study methods can be divided into three groups: genome-wide methylation extent analysis, gene-specific methylation analysis and new methylated sites screening. In this article, we introduced most existing DNA methlylation detecting techniques and summarized their characteristics at the same time.

Key words: Epigenetics, DNA methylation, methylation detecting technique