Long double-stranded RNAs (dsRNAs; typically >200 nt) can be used to silence the expression of target genes in a variety of organisms and cell types (e.g. worms fruit flies and plants). Upon introd ... [阅读全文]
Overview
We design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse Refseq transcript. The algorithm applies a set of ... [阅读全文]
The protocols listed here are for Drosophila cells in 6 well plates and our pre-aliquoted 384 well plates. RNAi experiments may be done in other size plates just scale up or down.
Adopted from Clemens ... [阅读全文]
1) Place gene of interest between T7 promoters in Òdouble T7Ó plamsid. (slightly more difficμlt alternative: Place gene of interest in hairpin/inverted repeat config ... [阅读全文]
RNA interference (RNAi) is a phenomenon in which the introduction of double-stranded RNA (dsRNA) into a diverse range of organisms and cell types causes degradation of the complementary mRNA (Figure 1 ... [阅读全文]
本文来自于哈佛大学医学院果蝇RNAi 筛选中心的经典实验方法，专门用于果蝇RNAi 实验方法。感谢哈佛大学医学院果蝇RNAi 筛选中心的支持！
A. Primer Designed dsRNA
B. Template Selection
C. PCR
D. In vitro RNA Transcription
E. dsRNA Purif ... [阅读全文]
RNA interference (RNAi ) by double stranded RNA (dsRNAs) molecules of approximately 20-25 nucleotides termed short interfering (siRNAs) is a powerful method for preventing the expression of a particul ... [阅读全文]
小分子干扰RNA (Small interfering RNA ，siRNA ) 是一种非常有效的工具，能够在包括哺乳动物细胞在内的多个体系中抑制特定基因的表达，从而研究某个基因的功能或者是相关信息。但是这种强有力的方法的难题之一是需要设计，合成siRNA s 和验证其效果，从而找到最有效的siRNA s 。这个过程需要花费相当的时间和经费。以化学法合成基因特异的siRNA s 为例，由于设计好的 ... [阅读全文]
RNA interference (RNAi ) by double stranded RNA (dsRNAs) molecules of approximately 20-25 nucleotides termed short interfering (siRNAs) is a powerful method for preventing the expression of a particul ... [阅读全文]
pSico Oligo Design
Click on the Mac OSX program pSicoOligomaker 1.5 to select and design oligos for pSico and plentilox3.7.
To operate it simply paste your sequence in the "sequence" window ... [阅读全文]
1.NCBIshRNA资源库
http://cgap.nci.nih.gov/RNAi
About RNAi on CGAP
The NCBI is part of the consortium supporting the preparation of human and mouse libraries containing RNAi constructs that target cance ... [阅读全文]
RNA i Glossary
Dicer - Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNA s. Dicer processes long dsRNA into siRNA of 21-23 nt.
Interferon - A small ... [阅读全文]
RNAi 实验中双链短RNA（dsRNA）制备过程，本实验方法来自于加州大学Jim教授实验，很权威！
Procedure for the Generation of dsRNA for use in RNAi
1. Design polymerase chain reaction (PCR ) primers that will amplify 600-800 bp of sequenc ... [阅读全文]
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